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A promising approach to bone tissue engineering lies
in the use of perfusion bioreactors where cells are seeded and
cultured on scaffolds under conditions of enhanced nutrient
supply and removal of metabolic products. Fluid flow alterations
can stimulate cell activity, making the engineering of tissue
more efficient. Most bioreactor systems are used to culture
cells on thin scaffold discs. In clinical use, however, bone substitutes
of large dimensions are needed. In this study, MG63
osteoblast-like cells were seeded on large porous PLA/glass
scaffolds with a custom developed perfusion bioreactor system.
Cells were seeded by oscillating perfusion of cell suspension
through the scaffolds. Applicable perfusion parameters for successful
cell seeding were determined by varying fluid flow velocity
and perfusion cycle number. After perfusion, cell seeding,
the cell distribution, and cell seeding efficiency were determined.
A fluid flow velocity of 5 mm/s had to be exceeded to
achieve a uniform cell distribution throughout the scaffold interior.
Cell seeding efficiencies of up to 50% were achieved.
Results suggested that perfusion cycle number influenced cell
seeding efficiency rather than fluid flow velocities. The cell
seeding conducted is a promising basis for further long term
cell culture studies in large porous scaffolds.
CitacióKoch, M. [et al.]. Perfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.. "Journal of biomedical materials research. Part A", 17 Desembre 2010, vol. 95, núm. 4, p. 1011-1018.