Biofunctionalization of titanium surfaces for osseintegration process improvement
Tipus de documentArticle
EditorInstitute Of Physics Publishing Ltd
Condicions d'accésAccés restringit per política de l'editorial
This study aims to improve the osseointegration of titanium implants through surface immobilization of peptides that induce a beneficial biological response. This was carried out biofunctionalizating titanium surfaces by silanization and subsequent covalent binding of a peptide with a sequence that promotes cell adhesion. Objective: The development of a new technique of immobilization of oligopeptides on the surface of titanium by using 3-chloropropyltrietoxisilane (CPTES) as bonding agent between the surface of titanium and the peptide. Materials and methods: A physicochemical characterization of the surfaces through the techniques of XPS, ToF-SIMS and contact angle was performed. Also cell adhesion studies have been conducted to evaluate in vitro biological response. Results: Through the process of silanization the titanium surface is completely covered with CPTES, which allows the subsequent accession of oligopeptides. The cell adhesion results show a higher cell adhesion and cell extension on biofunctionalized samples. Conclusions: We developed a system of covalent binding of oligopeptides on titanium surfaces that can modify the biological response of the attached cells.
CitacióSevilla, P. [et al.]. Biofunctionalization of titanium surfaces for osseintegration process improvement. "Journal of physics: conference series (online)", 01 Juny 2010, vol. 252, p. 1-6.