Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: A gene expression study using real-time RT-PCR
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Data publicació2011-03
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Abstract
Biomaterial surface properties, via alterations
in the adsorbed protein layer, and the presence of specific
functional groups can influence integrin binding specificity,
thereby modulating cell adhesion and differentiation processes.
The adsorption of fibronectin, a protein directly
involved in osteoblast adhesion to the extracellular matrix,
has been related to different physical and chemical properties
of biomaterial surfaces. This study used blasting
particles of different sizes and chemical compositions to
evaluate the response of MG63 osteoblast-like cells on
smooth and blasted titanium surfaces, with and without
fibronectin coatings, by means of real-time reverse
transcription-polymerase chain reaction (qRT-PCR) assays.
This response included (a) expression of the a5, av and a3
integrin subunits, which can bind to fibronectin through the
RGD binding site, and (b) expression of alkaline phosphatase
(ALP) and osteocalcin (OC) as cell-differentiation
markers. ALP activity and synthesis of OC were also tested.
Cells on SiC-blasted Ti surfaces expressed higher
amounts of the a5 mRNA gene than cells on Al2O3-blasted
Ti surfaces. This may be related to the fact that SiC-blasted
surfaces adsorbed higher amounts of fibronectin due to
their higher surface free energy and therefore provided a
higher number of specific cell-binding sites. Fn-coated Ti surfaces decreased a5 mRNA gene expression, by favoring
the formation of other integrins involved in adhesion over
a5b1. The changes in a5 mRNA expression induced by the
presence of fibronectin coatings may moreover influence
the osteoblast differentiation pathway, as fibronectin coatings
on Ti surfaces also decreased both ALP mRNA
expression and ALP activity after 14 and 21 days of cell
culture.
CitacióPegueroles, Marta [et al.]. Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: A gene expression study using real-time RT-PCR. "Journal of materials science. Materials in medicine", Març 2011, vol. 22, núm. 3, p. 617-627.
ISSN0957-4530
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