Fabrication of flexible interdigitated µ-electrodes (FIDµEs) for the development of a conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles
Visualitza/Obre
text complet (779,2Kb) (Accés restringit)
Sol·licita una còpia a l'autor
Què és aquest botó?
Aquest botó permet demanar una còpia d'un document restringit a l'autor. Es mostra quan:
- Disposem del correu electrònic de l'autor
- El document té una mida inferior a 20 Mb
- Es tracta d'un document d'accés restringit per decisió de l'autor o d'un document d'accés restringit per política de l'editorial
Cita com:
hdl:2117/12582
Tipus de documentArticle
Data publicació2010
Condicions d'accésAccés restringit per política de l'editorial
Tots els drets reservats. Aquesta obra està protegida pels drets de propietat intel·lectual i
industrial corresponents. Sense perjudici de les exempcions legals existents, queda prohibida la seva
reproducció, distribució, comunicació pública o transformació sense l'autorització del titular dels drets
Abstract
The present paper describes a simple and low-cost method for the fabrication of mechanically flexible interdigitated μ-electrodes (FIDμEs) and its application as immunosensor.
FIDμEs consist of two coplanar non-passivated interdigitated metallic μ-electrodes supported on a flexible transparent substrate, polyethylene naphthalate (PEN). Bioreagents deposition on PEN substrates becomes possible by depositing SiO2 on the electrodes surface (fingers and inter-digits space).
These FIDμEs were successfully applied for the development of a selective conductimetric immunosensor for the quantification of atrazine residues. The immunosensor has been demonstrated for detection of small amounts of atrazine, thanks to the use of immunoreagents specifically developed to detect this pesticide.
The detection method applied is based on the use of antibodies labelled with gold nanoparticles. The presence of these particles amplifies the conductive signal; hence the immunosensor response was quantified using simple and inexpensive DC measurements.
Immunochemical detection of the concentrations of atrazine is achieved by a competitive reaction which occurs before the inclusion of the labelled antibodies.
The immunosensor shows limits of detection in the order of 2–3 μg L−1, far below the maximum residue level (50 μg kg−1) established by EU for residues of atrazine as herbicide in the wine grapes and other foodstuff products.
CitacióValera, E.; Muñiz, D.; Rodriguez, A. Fabrication of flexible interdigitated µ-electrodes (FIDµEs) for the development of a conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles. "Microelectronic engineering", 2010, vol. 87, núm. 2, p. 167-173.
ISSN0167-9317
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
Angel_sdarticle ... vMicroelectornic-Elsev.pdf | text complet | 779,2Kb | Accés restringit |