Mostra el registre d'ítem simple
Modelling and parameter estimation of gene expression and cell growth in batch cultures
dc.contributor.author | Cubarsí Morera, Rafael |
dc.contributor.author | Corchero, José Luis |
dc.contributor.author | Vila, Pau |
dc.contributor.author | Villaverde, Antonio |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Matemàtica Aplicada IV |
dc.date.accessioned | 2011-04-12T14:12:44Z |
dc.date.available | 2011-04-12T14:12:44Z |
dc.date.created | 2000 |
dc.date.issued | 2000 |
dc.identifier.citation | Cubarsi, R. [et al.]. Modelling and parameter estimation of gene expression and cell growth in batch cultures. A: 3rd European Congress of Mathematics. "3rd European Congress of Mathematics". Barcelona: Carles Casacuberta, Josep Maria Font, Santiago Zarzuela, 2000, p. 1-13. |
dc.identifier.uri | http://hdl.handle.net/2117/12353 |
dc.description.abstract | Experimental procedure of CI857ts-controlled recombinant gene expression in bacterial batch cultures is mathematically modelled, and the corresponding minimum variance parameters are estimated from specific statistical or numerical methods, basically by using a global and recursive weighted least squares procedure under some constraints induced by the model. Moreover the numerical techniques proposed in this work act by accumulation of data coming from several runs of the experiment, so that more accuracy is obtained in the parameter estimation. In particular, for the production process, an extra-model parameter depending on an indicator vector is introduced for each run of the experiment in order to globalize the data. The analysis of obtained data leads to an integrated model for both cell growth and gene expression, which describes an asymmetric dynamics between culture growth and protein yield, and can serve to predict the maximal value of accumulated protein and the time required for it to be achieved at any stage of the preinducing cell growth. |
dc.format.extent | 13 p. |
dc.language.iso | eng |
dc.publisher | Carles Casacuberta, Josep Maria Font, Santiago Zarzuela |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Matemàtiques i estadística::Anàlisi numèrica::Modelització matemàtica |
dc.subject.lcsh | Mathematical models |
dc.subject.lcsh | Parameter estimation |
dc.subject.lcsh | Least squares |
dc.subject.lcsh | Numerical analysis |
dc.title | Modelling and parameter estimation of gene expression and cell growth in batch cultures |
dc.type | Conference report |
dc.subject.lemac | Models matemàtics |
dc.subject.lemac | Estimació paramètrica |
dc.subject.lemac | Mínims quadrats |
dc.subject.lemac | Anàlisi numèrica |
dc.contributor.group | Universitat Politècnica de Catalunya. gAGE - Grup d'Astronomia i Geomàtica |
dc.subject.ams | Classificació AMS::62 Statistics |
dc.subject.ams | Classificació AMS::92 Biology and other natural sciences |
dc.subject.ams | Classificació AMS::65 Numerical analysis |
dc.rights.access | Open Access |
local.identifier.drac | 2371297 |
dc.description.version | Postprint (published version) |
local.citation.author | Cubarsi, R.; Corchero, J.L.; Vila, P.; Villaverde, A. |
local.citation.contributor | 3rd European Congress of Mathematics |
local.citation.pubplace | Barcelona |
local.citation.publicationName | 3rd European Congress of Mathematics |
local.citation.startingPage | 1 |
local.citation.endingPage | 13 |