Mostra el registre d'ítem simple
Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
dc.contributor.author | Ciapetti, Gabriela |
dc.contributor.author | Pompo, Gemma di |
dc.contributor.author | Avnet, Sofia |
dc.contributor.author | Martini, Desirée |
dc.contributor.author | Díez Escudero, Anna |
dc.contributor.author | Montufar Jiménez, Edgar Benjamin |
dc.contributor.author | Ginebra Molins, Maria Pau |
dc.contributor.author | Baldini, Nicola |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.date.accessioned | 2017-05-12T10:34:08Z |
dc.date.available | 2017-05-12T10:34:08Z |
dc.date.issued | 2017-03-01 |
dc.identifier.citation | Ciapetti, G., Pompo, G., Avnet, S., Martini, D., Diez, A., Montufar, Edgar B., Ginebra, M.P., Baldini, N. Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates. "Acta biomaterialia", 1 Març 2017, vol. 50, p. 102-113. |
dc.identifier.issn | 1742-7061 |
dc.identifier.uri | http://hdl.handle.net/2117/104354 |
dc.description.abstract | The design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity. In this study, the adhesion and differentiation of human blood-derived osteoclast precursors (OCP) on two different micro-nanostructured biomimetic hydroxyapatite materials consisting in coarse (HA-C) and fine HA (HA-F) crystals, in comparison with sintered stoichiometric HA (sin-HA, reference material), were investigated. Osteoclasts were induced to differentiate by RANKL-containing supernatant using cell/substrate direct and indirect contact systems, and calcium (Ca++) and phosphorus (P5+) in culture medium were measured. We observed that OCP adhered to the experimental surfaces, and that osteoclast-like cells formed at a rate influenced by the micro- and nano-structure of HA, which also modulate extracellular Ca++. Qualitative differences were found between OCP on biomimetic HA-C and HA-F and their counterparts on plastic and sin-HA. On HA-C and HA-F cells shared typical features of mature osteoclasts, i.e. podosomes, multinuclearity, tartrate acid phosphatase (TRAP)-positive staining, and TRAP5b-enzyme release. However, cells were less in number compared to those on plastic or on sin-HA, and they did not express some specific osteoclast markers. In conclusion, blood-derived OCP are able to attach to biomimetic and sintered HA substrates, but their subsequent fusion and resorptive activity are hampered by surface micro-nano-structure. Indirect cultures suggest that fusion of OCP is sensitive to topography and to extracellular calcium. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Bone resorption |
dc.subject.lcsh | Hydroxyapatite |
dc.subject.lcsh | Osteoclasts |
dc.subject.lcsh | Topography |
dc.subject.other | Osteoclasts |
dc.subject.other | Hydroxyapatite |
dc.subject.other | Differentiation |
dc.subject.other | Topography |
dc.subject.other | Ionic exchange |
dc.subject.other | Bone resorption |
dc.title | Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates |
dc.type | Article |
dc.subject.lemac | Hidroxiapatita |
dc.subject.lemac | Osteopatia |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1016/j.actbio.2016.12.013 |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S174270611630681X |
dc.rights.access | Open Access |
local.identifier.drac | 20096346 |
dc.description.version | Preprint |
dc.contributor.covenantee | Istituto Ortopedico Rizzoli |
local.citation.author | Ciapetti, G.; Pompo, G.; Avnet, S.; Martini, D.; Diez, A.; Montufar, Edgar B.; Ginebra, M.P.; Baldini, N. |
local.citation.publicationName | Acta biomaterialia |
local.citation.volume | 50 |
local.citation.startingPage | 102 |
local.citation.endingPage | 113 |
Fitxers d'aquest items
Aquest ítem apareix a les col·leccions següents
-
Articles de revista [960]
-
Articles de revista [439]