GeNIUL, experts on viability PCR

Abstract

The standard PCR technique allows the rapid detection of microorganisms, difficult to detect by traditional culture methods. However, the control of samples through PCR has the drawback of false positives due to amplification of DNA/RNA of non-viable cells. This can be overcome by adding intercalating agents (EMA or PMA) to the sample, which selectively penetrates nonviable cells and blocks their DNA/RNA after a photo-activation process, preventing its subsequent amplification by PCR. This process is known as viability PCR (v-PCR). v-PCR has been used as an accurate, reliable and simple method to detect and quantify viable microorganism involved in environmental samples, food production process and clinical samples. GenIUL provides the reagents (EMA and PMA) and the PhAST Blue photo-activation system needed to carry out v-PCR, ensuring optimum and uniform photochemical reaction of intercalating agents prior to PCR