Modelling of metabolic reaction network for the development of an optimal nutrient cocktail for animal cell culture
Tutor / director / evaluatorJacobsen, Elling
Document typeMaster thesis (pre-Bologna period)
Rights accessRestricted access - confidentiality agreement
In a cell culture system, a lot of compounds are involved considering the ones contained in the medium (extracellular) and the intracellular environment. Studies of the different system behaviours adopted due to variations in the medium concentrations are typically based on empirical or statistical approaches when working with such an amount of compounds. In this paper is presented a method to develop a dynamical model of a cell culture system which can predict the evolution of the medium compounds and cell growth along the time for this kind of systems. The method has been implemented as a standardised tool that can be used for any kind of biological system when a pre-defined metabolic network and data of different states are available. In parallel to this project, a medium where the exact concentrations of all the compounds are known and should let the cells live properly have been developed by the KTH Division of Bioprocess. The system corresponding to the experiments carried out to develop the medium has been modelled using the proposed method. The results have been analysed and the conclusions, requirements and drawbacks of the model have been discussed.
ProvenanceAquest document conté originàriament altre material i/o programari no inclòs en aquest lloc web
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