Articles de revistahttp://hdl.handle.net/2117/37232024-03-28T14:14:12Z2024-03-28T14:14:12ZTear osmolarity and ocular changes in pterygiumJulio Morán, GemmaLluch Margarit, SaraPujol Vives, PereAlonso Perez, SilviaMerindano Encina, M. Doloreshttp://hdl.handle.net/2117/3270462021-11-14T10:47:21Z2020-07-20T06:16:24ZTear osmolarity and ocular changes in pterygium
Julio Morán, Gemma; Lluch Margarit, Sara; Pujol Vives, Pere; Alonso Perez, Silvia; Merindano Encina, M. Dolores
Purpose: To determine abnormalities in tear osmolarity (TO), tear function, and impression cytology in patients with pterygium and to assess the relationship between the variables. Methods: Thirty eyes from 30 patients with primary nasal pterygium and 30 eyes from 30 volunteers without ocular pathologies or dry eye symptoms were enrolled in the present study. TO test, tear ferning test, fluorescein tear breakup time, Schirmer test, and impression cytology of the conjunctiva were performed. Analysis of variance was applied for intergroup comparisons, and Pearson correlation was used to calculate the strength of relationships between the variables. A statistical significance level of P<0.05 was considered. Results: Pterygium patients had significantly higher TO, lower crystallization percentage, and lower goblet cell density (GCD) than control patients. A weak but significant negative correlation seems to exist between TO and crystallization percentage (r=-0.425, P<0.01) and between TO and GCD (r=-0.295, P<0.05). Conclusion: There is evidence to suggest that pterygium appears to induce unfavorable conditions of increasing TO that could trigger alterations in tear crystallization and GCD. Being aware of TO changes seems essential to understand the complex relationship among pterygium, tear film functions, and ocular surface changes.
2020-07-20T06:16:24ZJulio Morán, GemmaLluch Margarit, SaraPujol Vives, PereAlonso Perez, SilviaMerindano Encina, M. DoloresPurpose: To determine abnormalities in tear osmolarity (TO), tear function, and impression cytology in patients with pterygium and to assess the relationship between the variables. Methods: Thirty eyes from 30 patients with primary nasal pterygium and 30 eyes from 30 volunteers without ocular pathologies or dry eye symptoms were enrolled in the present study. TO test, tear ferning test, fluorescein tear breakup time, Schirmer test, and impression cytology of the conjunctiva were performed. Analysis of variance was applied for intergroup comparisons, and Pearson correlation was used to calculate the strength of relationships between the variables. A statistical significance level of P<0.05 was considered. Results: Pterygium patients had significantly higher TO, lower crystallization percentage, and lower goblet cell density (GCD) than control patients. A weak but significant negative correlation seems to exist between TO and crystallization percentage (r=-0.425, P<0.01) and between TO and GCD (r=-0.295, P<0.05). Conclusion: There is evidence to suggest that pterygium appears to induce unfavorable conditions of increasing TO that could trigger alterations in tear crystallization and GCD. Being aware of TO changes seems essential to understand the complex relationship among pterygium, tear film functions, and ocular surface changes.Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplificationFittipaldi Gustavino, MarianaNocker, AndreasCodony Iglesias, Franceschttp://hdl.handle.net/2117/1258932020-07-23T20:38:21Z2018-12-17T19:11:37ZProgress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification
Fittipaldi Gustavino, Mariana; Nocker, Andreas; Codony Iglesias, Francesc
The ideal scenario in most applications of microbial diagnostics is that only viable cells are detected. Bacteria were traditionally considered viable when they could be cultured, whereas today's viability concept tends to be alternatively based on the presence of some form of metabolic activity, a positive energy status, responsiveness, detection of RNA transcripts that tend to degrade rapidly after cell death, or of an intact membrane. The latter criterion, although conservative, was the focus of one of the most successful recent approaches to detect viable cells in combination with DNA amplification techniques. The technology is based on sample treatment with the photoactivatable, and cell membrane impermeant, nucleic acid intercalating dyes ethidium monoazide (EMA) or propidium monoazide (PMA) followed by light exposure prior to extraction of DNA and amplification. Light activation of DNA-bound dye molecules results in irreversible DNA modification and subsequent inhibition of its amplification. Sample pretreatment with viability dyes has so far been mainly used in combination with PCR (leading to the term viability PCR, v-PCR), and increasingly with isothermal amplification method. The principle is not limited to bacteria, but has also successfully been applied to fungi, protozoa and viruses. Despite the success of the method, some practical limitations have been identified, especially when applied to environmental samples. In part they can be minimized by choice of experimental parameters and conditions adequate for a particular sample. This review summarizes current knowledge and presents aspects which are important when designing experiments employing viability dyes.
2018-12-17T19:11:37ZFittipaldi Gustavino, MarianaNocker, AndreasCodony Iglesias, FrancescThe ideal scenario in most applications of microbial diagnostics is that only viable cells are detected. Bacteria were traditionally considered viable when they could be cultured, whereas today's viability concept tends to be alternatively based on the presence of some form of metabolic activity, a positive energy status, responsiveness, detection of RNA transcripts that tend to degrade rapidly after cell death, or of an intact membrane. The latter criterion, although conservative, was the focus of one of the most successful recent approaches to detect viable cells in combination with DNA amplification techniques. The technology is based on sample treatment with the photoactivatable, and cell membrane impermeant, nucleic acid intercalating dyes ethidium monoazide (EMA) or propidium monoazide (PMA) followed by light exposure prior to extraction of DNA and amplification. Light activation of DNA-bound dye molecules results in irreversible DNA modification and subsequent inhibition of its amplification. Sample pretreatment with viability dyes has so far been mainly used in combination with PCR (leading to the term viability PCR, v-PCR), and increasingly with isothermal amplification method. The principle is not limited to bacteria, but has also successfully been applied to fungi, protozoa and viruses. Despite the success of the method, some practical limitations have been identified, especially when applied to environmental samples. In part they can be minimized by choice of experimental parameters and conditions adequate for a particular sample. This review summarizes current knowledge and presents aspects which are important when designing experiments employing viability dyes.Utilidad de las técnicas de biología molecular para el control analítico de la calidad microbiológica de las aguasCodony Iglesias, FrancescPérez, Leonardo MartínMorató Farreras, JordiDomínguez Gual, María Carolinahttp://hdl.handle.net/2117/881672023-03-01T17:26:04Z2016-06-20T11:58:19ZUtilidad de las técnicas de biología molecular para el control analítico de la calidad microbiológica de las aguas
Codony Iglesias, Francesc; Pérez, Leonardo Martín; Morató Farreras, Jordi; Domínguez Gual, María Carolina
2016-06-20T11:58:19ZCodony Iglesias, FrancescPérez, Leonardo MartínMorató Farreras, JordiDomínguez Gual, María CarolinaPrevalence and concentration of non-tuberculous mycobacteria in cooling towers by means of quantitative PCR: a prospective studyAdrados Ruiz, BarbaraJulian, EstherCodony Iglesias, FrancescTorrents, EduardLuquin, MarinaMorató Farreras, Jordihttp://hdl.handle.net/2117/870242020-07-23T22:33:54Z2016-05-12T14:16:48ZPrevalence and concentration of non-tuberculous mycobacteria in cooling towers by means of quantitative PCR: a prospective study
Adrados Ruiz, Barbara; Julian, Esther; Codony Iglesias, Francesc; Torrents, Eduard; Luquin, Marina; Morató Farreras, Jordi
2016-05-12T14:16:48ZAdrados Ruiz, BarbaraJulian, EstherCodony Iglesias, FrancescTorrents, EduardLuquin, MarinaMorató Farreras, JordiA new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilmsPérez, Leonardo MartínÁlvarez, B.L.Codony Iglesias, FrancescFittipaldi Gustavino, MarianaAdrados Ruiz, BarbaraPeñuela, GustavoMorató Farreras, Jordihttp://hdl.handle.net/2117/870182023-03-01T15:56:11Z2016-05-12T13:34:11ZA new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilms
Pérez, Leonardo Martín; Álvarez, B.L.; Codony Iglesias, Francesc; Fittipaldi Gustavino, Mariana; Adrados Ruiz, Barbara; Peñuela, Gustavo; Morató Farreras, Jordi
2016-05-12T13:34:11ZPérez, Leonardo MartínÁlvarez, B.L.Codony Iglesias, FrancescFittipaldi Gustavino, MarianaAdrados Ruiz, BarbaraPeñuela, GustavoMorató Farreras, JordiDetection of Catabacter hongkongensis in polluted European water samplesCodony Iglesias, FrancescAdrados Ruiz, BarbaraPérez, Leonardo MartínFittipaldi Gustavino, MarianaMorató Farreras, Jordihttp://hdl.handle.net/2117/870042023-03-08T11:35:40Z2016-05-12T11:25:51ZDetection of Catabacter hongkongensis in polluted European water samples
Codony Iglesias, Francesc; Adrados Ruiz, Barbara; Pérez, Leonardo Martín; Fittipaldi Gustavino, Mariana; Morató Farreras, Jordi
2016-05-12T11:25:51ZCodony Iglesias, FrancescAdrados Ruiz, BarbaraPérez, Leonardo MartínFittipaldi Gustavino, MarianaMorató Farreras, JordiUna tarea para evaluar la agudeza visual dinámica y una valoración de la estabilidad de sus medicionesQuevedo Junyent, Luisa JesúsAznar Casanova, José AntonioMerindano Encina, M. DoloresSolé Fortó, Joanhttp://hdl.handle.net/2117/866562021-05-20T08:10:47Z2016-05-05T15:09:16ZUna tarea para evaluar la agudeza visual dinámica y una valoración de la estabilidad de sus mediciones
Quevedo Junyent, Luisa Jesús; Aznar Casanova, José Antonio; Merindano Encina, M. Dolores; Solé Fortó, Joan
2016-05-05T15:09:16ZQuevedo Junyent, Luisa JesúsAznar Casanova, José AntonioMerindano Encina, M. DoloresSolé Fortó, JoanA novel computer software for the evaluation of dynamic visual acuityQuevedo Junyent, Luisa JesúsAznar Casanova, José AntonioMerindano Encina, M. DoloresCardona Torradeflot, GenísSolé Fortó, Joanhttp://hdl.handle.net/2117/855602021-05-20T14:22:05Z2016-04-12T12:28:27ZA novel computer software for the evaluation of dynamic visual acuity
Quevedo Junyent, Luisa Jesús; Aznar Casanova, José Antonio; Merindano Encina, M. Dolores; Cardona Torradeflot, Genís; Solé Fortó, Joan
2016-04-12T12:28:27ZQuevedo Junyent, Luisa JesúsAznar Casanova, José AntonioMerindano Encina, M. DoloresCardona Torradeflot, GenísSolé Fortó, JoanThreshold to predict astigmatism reduction after pterygium excisionPujol Vives, PereJulio Morán, Gemmade Carvalho Mendes Castanheira, Amélia MariaKostov, Belchin AdriyanovPujol Vives, Perehttp://hdl.handle.net/2117/278422023-02-19T10:42:15Z2015-05-08T11:20:38ZThreshold to predict astigmatism reduction after pterygium excision
Pujol Vives, Pere; Julio Morán, Gemma; de Carvalho Mendes Castanheira, Amélia Maria; Kostov, Belchin Adriyanov; Pujol Vives, Pere
Purpose. To compare corneal astigmatism after pterygium excision, using limbal-conjunctival autograft (LCA) with and without mitomycin C (MMC) and to establish a threshold for postoperative astigmatism reduction.; Methods. Sixty-eight eyes with primary pterygium were consecutively sampled and assigned to LCA with MMC (MMC+) or LCA without MMC (MMC-). Corneal lesion length, corneal lesion area, preoperative corneal astigmatism (PRCA), and postoperative corneal astigmatism (POCA) at 1, 3, and 6 months were assessed.; Results. Thirty-six men and 24 women (60 eyes), aged between 24 and 65 years (mean +/- SD, 41 +/- 8.2 years), completed the study. Thirty-one eyes were included in the MMC+ group and 29 were in the MMC- group. Both groups showed astigmatism reduction (p < 0.05; paired, two-tailed t test) 1 month after the surgery and remained stable. No differences were found between groups in corneal lesion length, corneal lesion area, or astigmatism results at the four time points (p 9 0.05, independent t test). Preoperative corneal astigmatism showed a significant correlation with POCA at 6 months (r = 0.529; p < 0.01). According to receiver operating characteristic curve analysis, the better threshold for astigmatism reduction with the surgery was 1.05 diopters of PRCA, with 82.5% sensitivity and 80.5% specificity.; Conclusions. Both surgical procedures could have similar astigmatism results. Pterygium patients with more than 1.05 diopters of PRCA could reduce it after the surgery. Direct relationship between PRCA and POCA revealed that postoperative astigmatism reduction was partial. Therefore, for minimizing final astigmatism, preoperative values should be slightly above the threshold.
2015-05-08T11:20:38ZPujol Vives, PereJulio Morán, Gemmade Carvalho Mendes Castanheira, Amélia MariaKostov, Belchin AdriyanovPujol Vives, PerePurpose. To compare corneal astigmatism after pterygium excision, using limbal-conjunctival autograft (LCA) with and without mitomycin C (MMC) and to establish a threshold for postoperative astigmatism reduction.; Methods. Sixty-eight eyes with primary pterygium were consecutively sampled and assigned to LCA with MMC (MMC+) or LCA without MMC (MMC-). Corneal lesion length, corneal lesion area, preoperative corneal astigmatism (PRCA), and postoperative corneal astigmatism (POCA) at 1, 3, and 6 months were assessed.; Results. Thirty-six men and 24 women (60 eyes), aged between 24 and 65 years (mean +/- SD, 41 +/- 8.2 years), completed the study. Thirty-one eyes were included in the MMC+ group and 29 were in the MMC- group. Both groups showed astigmatism reduction (p < 0.05; paired, two-tailed t test) 1 month after the surgery and remained stable. No differences were found between groups in corneal lesion length, corneal lesion area, or astigmatism results at the four time points (p 9 0.05, independent t test). Preoperative corneal astigmatism showed a significant correlation with POCA at 6 months (r = 0.529; p < 0.01). According to receiver operating characteristic curve analysis, the better threshold for astigmatism reduction with the surgery was 1.05 diopters of PRCA, with 82.5% sensitivity and 80.5% specificity.; Conclusions. Both surgical procedures could have similar astigmatism results. Pterygium patients with more than 1.05 diopters of PRCA could reduce it after the surgery. Direct relationship between PRCA and POCA revealed that postoperative astigmatism reduction was partial. Therefore, for minimizing final astigmatism, preoperative values should be slightly above the threshold.Methods for the preparation of doxycycline-loaded phb micro- and nano-spheres.Gil Mur, Francisco JavierRodríguez Contreras, Alejandra MaríaGinebra Molins, Maria PauCanal Barnils, CristinaCalafell Monfort, MargaritaMarqués Calvo, M. Soledadhttp://hdl.handle.net/2117/275482021-05-20T08:01:07Z2015-04-23T10:51:37ZMethods for the preparation of doxycycline-loaded phb micro- and nano-spheres.
Gil Mur, Francisco Javier; Rodríguez Contreras, Alejandra María; Ginebra Molins, Maria Pau; Canal Barnils, Cristina; Calafell Monfort, Margarita; Marqués Calvo, M. Soledad
Natural and synthetic biodegradable polymers have been investigated for controlled drug
release. Poly(3-hydroxybutyrate) can be produced by bacteria and is remarkable for this
application due to its excellent biocompatibility and biodegradability. The objective of this
work was to study different drug-entrapment and emulsification methods for the obtaining
of doxycycline-loaded PHB micro- and nano-spheres. The micro-/nano-particles were
prepared by polymer precipitation via dialysis, simple emulsion (O/W) or multiple emulsion
(W1/O/W2) applying solvent evaporation in the last two cases. This was carried out
either by ultrasonication, dripping and/or high speed stirring. Different processing conditions
were varied in order to evaluate their influence on morphology, size, and drug entrapment
capabilities. The highest drug loading was obtained by single emulsion with high
speed stirring. In the case of multiple emulsion, the combination of ultrasound with high
speed stirring resulted in the most elevate process yield and drug loading capability.
2015-04-23T10:51:37ZGil Mur, Francisco JavierRodríguez Contreras, Alejandra MaríaGinebra Molins, Maria PauCanal Barnils, CristinaCalafell Monfort, MargaritaMarqués Calvo, M. SoledadNatural and synthetic biodegradable polymers have been investigated for controlled drug
release. Poly(3-hydroxybutyrate) can be produced by bacteria and is remarkable for this
application due to its excellent biocompatibility and biodegradability. The objective of this
work was to study different drug-entrapment and emulsification methods for the obtaining
of doxycycline-loaded PHB micro- and nano-spheres. The micro-/nano-particles were
prepared by polymer precipitation via dialysis, simple emulsion (O/W) or multiple emulsion
(W1/O/W2) applying solvent evaporation in the last two cases. This was carried out
either by ultrasonication, dripping and/or high speed stirring. Different processing conditions
were varied in order to evaluate their influence on morphology, size, and drug entrapment
capabilities. The highest drug loading was obtained by single emulsion with high
speed stirring. In the case of multiple emulsion, the combination of ultrasound with high
speed stirring resulted in the most elevate process yield and drug loading capability.