Surface modifications of silicon nitride for cellular biosensor applications
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hdl:2117/8056
Tipus de documentArticle
Data publicació2008-01
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Abstract
Thin films of silicon nitride (Si3N4) can be used
in several kinds of micro-sized biosensors as a material to
monitor fine environmental changes related to the process
of bone formation in vitro. We found however that Si3N4
does not provide optimal conditions for osseointegration as
osteoblast-like MG-63 cells tend to detach from the surface
when cultured over confluence. Therefore Si3N4 was
modified with self-assembled monolayers bearing functional
end groups of primary amine (NH2) and carboxyl
(COOH) respectively. Both these modifications enhanced
the interaction with confluent cell layers and thus improve
osseointegration over Si3N4. Furthermore it was observed
that the NH2 functionality increased the adsorption of
fibronectin (FN), promoted cell proliferation, but delayed
the differentiation. We also studied the fate of pre-adsorbed
and secreted FN from cells to learn more about the impact
of above functionalities for the development of provisional
extracellular matrix on materials interface. Taken together
our data supports that Si3N4 has low tissue integration but
good cellular biocompatibility and thus is appropriate in
cellular biosensor applications such as the ion-sensitive
field effect transistor (ISFET). COOH and NH2 chemistries
generally improve the interfacial tissue interaction with the
sensor and they are therefore suitable substrates for monitoring
cellular growth or matrix deposition using electrical
impedance spectroscopy.
CitacióGustavsson, J. [et al.]. Surface modifications of silicon nitride for cellular biosensor applications. "Journal of materials science. Materials in medicine", Gener 2008, vol. 19, núm. 4, p. 1839-1850.
ISSN0957-4530
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SurfacemodfSi3N4.pdf | 865,3Kb | Accés restringit |