Role of ECM/peptide coatings on SDF-1a triggered mesenchymal stromal cell migration from microcarriers for cell therapy
Visualitza/Obre
10.1016/j.actbio.2015.02.008
Inclou dades d'ús des de 2022
Cita com:
hdl:2117/28053
Tipus de documentArticle
Data publicació2015-05-01
Condicions d'accésAccés obert
Llevat que s'hi indiqui el contrari, els
continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
:
Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
Many cell therapies rely on the ability of mesenchymal stromal cells (MSCs) to diffuse and localize throughout the target tissue-such as tumoral and ischemic tissues-, in response to specific cytokine signals, rather than being concentrated at the site of implantation. Therefore, it is fundamental to engineer biomaterial carriers as reservoirs, from which cells can migrate, possibly in a controlled manner. In this work, microcarriers (µCs) made of polylactic acid are characterized as MSC delivery vehicles capable of modulating key chemotactic pathways. The effect of different functionalization strategies on MSC migratory behavior from the µCs is studied in vitro in relation to SDF-1a/CXCR4 axis,-a major actor in MSC recruitment, chemotaxis and homing. Collagen and arginine-glycine-aspartic acid (RGD) peptides were either covalently grafted or physisorbed on µC surface. While stable covalent modifications promoted better cell adhesion and higher proliferation compared to physisorption, the functionalization method of the µCs also affected the cells migratory behavior in response to SDF-1a (CXCL12) stimulation. Less stable coatings (physisorbed) showed sensibly higher number of migrating cells than covalent collagen/RGD coatings. The combination of physic-chemical cues provided by protein/peptide functionalization and stimuli induced by 3D culture on µCs improved MSC expression of CXCR4, and exerted a control over cell migration, a condition suitable to promote cell homing after transplantation in vivo. These are key findings to highlight the impact of surface modification approaches on chemokine-triggered cell release, and allow designing biomaterials for efficient and controlled cell delivery to damaged tissues.
CitacióLevato, R. [et al.]. Role of ECM/peptide coatings on SDF-1a triggered mesenchymal stromal cell migration from microcarriers for cell therapy. "Acta biomaterialia", 01 Maig 2015, vol. 18, p. 59-67.
ISSN1742-7061
Versió de l'editorhttp://www.sciencedirect.com/science/article/pii/S1742706115000719
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
LevatoR-actabio2015.docx | Article | 2,470Mb | Microsoft Word 2007 | Visualitza/Obre |