Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium
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Data publicació2013-04-27
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Abstract
In bone tissue engineering, the composition of the ionic extracellular environment (IEE) can determine
both cellular fate and a biomaterial
’
s development and performance. Therefore, precise control of the
IEE and a perfect understanding of the dynamic changes that it can be subject to due to cellular activity
ishighlydesired.Toachievethis,weinitiallymonito
redhowtwostandardosteoblast-likecellmodelsthat
expressedeitherhighorlowalkalinephosphataseactivity-SAOS-2andMG63cells,respectively-affected
the extracellular concentrations of calcium and phosphate during long-term cultures. It was observed
that cellular in
fl
uence onthe IEE varied greatly between the two models and could be linked to the capac-
ity of cells to deposit calcium in the extracellular matri
x. Miniaturized ion-selective electrodes that could
allow for real-time monitoring ofcalcium in aminimally invasive waywere then constructed.The electro-
des were characterized in standard
in vitro
cell culture environments, prior to being successfully applied
for periods of 24h, to record the dynamics of cell-induced deposition of calcium in the extracellular
matrix, while using osteogenic media of either high or low concentrations of phosphate. As a result, this
study provides the background and technological means for the non-destructive evaluation of the IEE
in vitro
and allows for the optimization and development of better models of bone tissue construction
CitacióGustavsson, J.; Planell, J.; Engel, E. Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium. "Journal of tissue engineering and regenerative medicine", 27 Abril 2013, vol. 7, núm. 8, p. 609-620.
ISSN1932-6254
Versió de l'editorhttp://onlinelibrary.wiley.com/doi/10.1002/term.550/abstract
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