Modular polylactic acid microparticle-based scaffolds prepared via microfluidic emulsion/solvent displacement process: Fabrication, characterization, and in vitro mesenchymal stem cells interaction study
Visualitza/Obre
modular.pdf.pdf (1,474Mb) (Accés restringit)
Sol·licita una còpia a l'autor
Què és aquest botó?
Aquest botó permet demanar una còpia d'un document restringit a l'autor. Es mostra quan:
- Disposem del correu electrònic de l'autor
- El document té una mida inferior a 20 Mb
- Es tracta d'un document d'accés restringit per decisió de l'autor o d'un document d'accés restringit per política de l'editorial
Cita com:
hdl:2117/19029
Tipus de documentArticle
Data publicació2012-08-31
Condicions d'accésAccés restringit per política de l'editorial
Llevat que s'hi indiqui el contrari, els
continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
:
Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
The present study reports a novel approach for the
design and fabrication of polylactic acid (PLA) microparticle-
based scaffolds with microstructural properties suitable for
bone and cartilage regeneration. Macroporous PLA scaffolds
with controlled shape were fabricated by means of a semicon-
tinuous process involving (1) microfluidic emulsification of a
PLA/ethyl lactate solution (5% w/v) in a span 80/paraffin oil so-
lution (3% v/v) followed by (2) particles coagulation/assembly
in an acetone/water solution for the development of a continu-
ous matrix. Porous scaffolds prepared from particles with
monomodal or bimodal size distribution, overall porosity
ranges from 93 to 96%, interparticles porosity from 41 to 54%,
and static compression moduli from 0.3 to 1.4 MPa were man-
ufactured by means of flow rate modulation of of the continu-
ous phase during emulsion. The biological response of the
scaffolds was assessed
in vitro
by using bone marrow-derived
rat mesenchymal stem cells (MSCs). The results demonstrated
the ability of the scaffolds to support the extensive and uni-
form three-dimensional adhesion, colonization, and prolifera-
tion of MSCs within the entire construct
CitacióSalerno, A. [et al.]. Modular polylactic acid microparticle-based scaffolds prepared via microfluidic emulsion/solvent displacement process: Fabrication, characterization, and in vitro mesenchymal stem cells interaction study. "Journal of biomedical materials research. Part A", 31 Agost 2012, vol. 101, núm. 3, p. 720-732.
ISSN1549-3296
Versió de l'editorhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34374/full
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
modular.pdf.pdf | 1,474Mb | Accés restringit |