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Citació: Koch, M. [et al.]. Perfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.. "Journal of biomedical materials research. Part A", 17 Desembre 2010, vol. 95, núm. 4, p. 1011-1018.
Títol: Perfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.
Autor: Koch, M.A.; Vrij, E.J.; Engel López, Elisabeth Veure Producció científica UPC; Planell Estany, Josep Anton Veure Producció científica UPC; Lacroix, Damien Jerome Veure Producció científica UPC
Data: 17-des-2010
Tipus de document: Article
Resum: A promising approach to bone tissue engineering lies in the use of perfusion bioreactors where cells are seeded and cultured on scaffolds under conditions of enhanced nutrient supply and removal of metabolic products. Fluid flow alterations can stimulate cell activity, making the engineering of tissue more efficient. Most bioreactor systems are used to culture cells on thin scaffold discs. In clinical use, however, bone substitutes of large dimensions are needed. In this study, MG63 osteoblast-like cells were seeded on large porous PLA/glass scaffolds with a custom developed perfusion bioreactor system. Cells were seeded by oscillating perfusion of cell suspension through the scaffolds. Applicable perfusion parameters for successful cell seeding were determined by varying fluid flow velocity and perfusion cycle number. After perfusion, cell seeding, the cell distribution, and cell seeding efficiency were determined. A fluid flow velocity of 5 mm/s had to be exceeded to achieve a uniform cell distribution throughout the scaffold interior. Cell seeding efficiencies of up to 50% were achieved. Results suggested that perfusion cycle number influenced cell seeding efficiency rather than fluid flow velocities. The cell seeding conducted is a promising basis for further long term cell culture studies in large porous scaffolds.
ISSN: 1549-3296
URI: http://hdl.handle.net/2117/14595
DOI: 10.1002/jbm.a.32927
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