DSpace DSpace UPC
 Català   Castellano   English  

E-prints UPC >
Altres >
Enviament des de DRAC >

Empreu aquest identificador per citar o enllaçar aquest ítem: http://hdl.handle.net/2117/13714

Arxiu Descripció MidaFormat
journal.pone.0023708.pdf727,53 kBAdobe PDFThumbnail
Veure/Obrir

Citació: Llech, A. [et al.]. Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes. "PLoS ONE Journal Information", 29 Agost 2011, vol. 6, núm. 8.
Títol: Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes
Autor: Llach, Anna; Molina, Cristina E.; Álvarez Lacalle, Enrique Veure Producció científica UPC; Tort, Lluis; Benítez Iglesias, Raúl Veure Producció científica UPC; Hove-Madsen, Leif
Data: 29-ago-2011
Tipus de document: Article
Resum: Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.4560.03 times the baseline fluorescence and the time to half maximal decay of sparks was 2763 ms. Spark frequency was 0.88 sparks mm21 min21 while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F0) from 1.7760.17 to 1.1260.18 (p=0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.660.3 min21 to 8.162.0 min21, p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.
ISSN: 1932-6203
URI: http://hdl.handle.net/2117/13714
DOI: 10.1371/journal.pone.0023708
Versió de l'editor: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023708
Apareix a les col·leccions:Altres. Enviament des de DRAC
NOLIN - Física No-Lineal i Sistemes Fora de l'Equilibri. Articles de revista
Departament de Física Aplicada. Articles de revista
Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial. Articles de revista
Comparteix:


Stats Mostra les estadístiques d'aquest ítem

SFX Query

Tots els drets reservats. Aquesta obra està protegida pels drets de propietat intel·lectual i industrial corresponents. Sense perjudici de les exempcions legals existents, queda prohibida la seva reproducció, distribució, comunicació pública o transformació sense l'autorització del titular dels drets.

Per a qualsevol ús que se'n vulgui fer no previst a la llei, dirigiu-vos a: sepi.bupc@upc.edu

 

Valid XHTML 1.0! Programari DSpace Copyright © 2002-2004 MIT and Hewlett-Packard Comentaris
Universitat Politècnica de Catalunya. Servei de Biblioteques, Publicacions i Arxius