During its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only ...

Gradients of signaling pathways within the intestinal stem cell (ISC) niche are instrumental for cellular compartmentalization and tissue function, yet how are they sensed by the epithelium is still not fully understood. ...

We report our observations of the transient self-narrowing of light beams mediated by dominant Kerr nonlinearities and two-photon absorption in a bulk potassium titanyl phosphate crystal near conditions for second-harmonic ...

Due to relatively high powers used in STED, biological samples may be affected by the illumination in the process of image acquisition. Similarly, the performance of the system may be limited by the sample itself. Optimization ...

Imaging molecular structures separated by distances of a few nanometers still represents a complex challenge. Moreover, it is normally restricted to observations on thin (few micrometers) samples. In this work, we rotate ...

Live microscopy techniques i.e., differential interference contrast, confocal microscopy, etc. have enabled the understanding of the mechanisms involved in cells and tissue formation. In long-term studies, special ...

We outline criteria for fast and accurate acquisition of collinear FROG (CFROG) trace and how it can be transformed into the more traditional noncollinear FROG trace. The CFROG has an intrinsically simple geometry that ...