• Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms 

    Loza Álvarez, Pablo; Artigas García, David; Santos, Susana I.C.O.; Aviles Espinosa, Rodrigo; Filippidis, George; Hamilton, Craig; Malcolm, Graeme; Weingarten, Kurt J.; Südmeyer, Thomas; Barbarin, Yohan; Keller, Ursula (2011-03-01)
    Article
    Accés restringit per política de l'editorial
    We present a portable ultrafast Semiconductor Disk Laser (SDL) (or vertical extended cavity surface emitting laser—VECSELs), to be used for nonlinear microscopy. The SDL is modelocked using a quantum-dot semiconductor ...
  • Decoupled illumination detection in light sheet microscopy for fast volumetric imaging 

    OLARTE, Omar Eduardo; Andilla, Jordi; Artigas García, David; Loza Álvarez, Pablo (Optical Society of American (OSA), 2015-08-04)
    Article
    Accés restringit per política de l'editorial
    Current microscopy demands the visualization of large threedimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet ...
  • Depth aberrations characterization in linear and nonlinear microscopy schemes using a Shack-Hartmann wavefront sensor 

    Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo (2012)
    Text en actes de congrés
    Accés restringit per política de l'editorial
    The performance of imaging devices such as linear and nonlinear microscopes (NLM) can be limited by the optical properties of the imaged sample. Such an important aspect has already been described using theoretical models ...
  • Effect of molecular organization on the image histograms of polarization SHG microscopy 

    Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Loza Álvarez, Pablo; Artigas García, David (2012)
    Article
    Accés obert
    Based on its polarization dependency, second harmonic generation (PSHG) microscopy has been proven capable to structurally characterize molecular architectures in different biological samples. By exploiting this polarization ...
  • Estimating the helical pitch angle of amylopectin in starch using polarization second harmonic generation microscopy 

    Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Loza Álvarez, Pablo; Artigas García, David (2010-08)
    Article
    Accés restringit per política de l'editorial
    Starch granules are among the brightest natural second harmonic generation (SHG) converters. They basically consist of amylose and amylopectin molecules and the source of the SHG signal is still undetermined. In the ...
  • Fast monitoring of in-vivo conformational changes in myosin using single scan polarization- SHG microscopy 

    Psilodimitrakopoulos, Sotiris; Loza Álvarez, Pablo; Artigas García, David (2014-12-01)
    Article
    Accés restringit per política de l'editorial
    Fast imaging of molecular changes under high-resolution and label-free conditions are essential for understanding in-vivo processes, however, current techniques are not able to monitor such changes in real time. Polarization ...
  • Imaging amylopectin's order in starch using 3-dimensional polarization SHG 

    Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Artigas García, David; Loza Álvarez, Pablo (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2011)
    Text en actes de congrés
    Accés restringit per política de l'editorial
    In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and ...
  • Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy 

    Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Olarte, Omar E.; Nieto, Marta; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo (2011-11-01)
    Article
    Accés obert
    We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear ...
  • Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis 

    Aviles Espinosa, Rodrigo; Santos, Susana I.C.O.; Brodschelm, Andreas; Kaenders, Wilhelm G.; Alonso Ortega, Cesar; Artigas García, David; Loza Álvarez, Pablo (2010-08)
    Article
    Accés obert
    Live microscopy techniques i.e., differential interference contrast, confocal microscopy, etc. have enabled the understanding of the mechanisms involved in cells and tissue formation. In long-term studies, special ...